%0 Journal Article %T Influence of hydrological fluxes on the structure of nitrate-reducing bacteria communities in a peatland %+ Géosciences Rennes (GR) %+ Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO) %A Bougon, Nolwenn %A Aquilina, Luc %A Briand, Marie-Paule %A Coedel, S. %A Vandenkoornhuyse, Philippe %< avec comité de lecture %Z PL02278 %@ 0038-0717 %J Soil Biology and Biochemistry %I Elsevier %V 41 %N 6 %P 1289–1300 %8 2009 %D 2009 %R 10.1016/j.soilbio.2009.03.015 %K Microbial diversity %K environmental changes %K hydrological flowpaths %K denitrification %K nitrate reducers %K narG %K T-RFLP %Z Sciences of the Universe [physics]/Earth Sciences/HydrologyJournal articles %X Factors influencing nitrate dynamics and nitrate-reducing bacteria in peat soil in the field, were investigated in laboratory experiments. A previous study had indicated that the on-site effects of redox conditions and nutrient fluxes on microbial activity were influenced by hydrological conditions. However, the laboratory experiments indicated that peat samples from sites under different hydrological regimes exhibited different microbial activities independently of oxygenation conditions. The effects of redox conditions and nutrient fluxes (i.e. influence of NO3 and O2 concentration) on the nitrate reducer community were therefore assessed. Microbial community structures in peat samples from sites under different hydrologic regimes were compared using Terminal-Restriction Fragment Length Polymorphism diversity signatures of the narG gene. This gene encodes the catalytic subunit of the nitrate reductase. Unexpectedly, the nitrate reducer communities were very similar at the beginning of the experiment whatever the peatland soil analysed. However, a strong structuration and divergence within the nitrate reducer communities, that was site-dependent, was evident after 76 h of incubation. These modifications within the microbial communities seemed to be due to differences in peat saturation at the sampling sites resulting from the different hydrological regimes. Of the forcing variables tested, oxygenation had a slight effect on the composition of the nitrate-reducers' community whereas nitrate addition had no effect. This study shows that a physical constraint such as hydrological regime might be considered important in microbial community composition. %G English %L insu-00392810 %U https://insu.hal.science/insu-00392810 %~ INSU %~ UNIV-RENNES1 %~ UR2-HB %~ CNRS %~ GR %~ OSUR %~ GIP-BE %~ ECOBIO %~ ECOBIO-RBPE %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDLM %~ UR1-SDLMJONCH %~ UR1-SDV %~ ECOBIO-DYNAMO %~ GR-DIMENV %~ UNIV-RENNES2 %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ INRAE %~ UR1-ENV %~ GR-ET