BSA and lysozyme adsorption on homoionic montmorillonite: Influence of the interlayer cation - INSU - Institut national des sciences de l'Univers Access content directly
Journal Articles Applied Clay Science Year : 2014

BSA and lysozyme adsorption on homoionic montmorillonite: Influence of the interlayer cation

Abstract

This study aims to investigate the influence of interlayer cation alkali metal series (Li+, Na+, K+, Rb+, Cs+) and alkali earth metal (Mg2 +, Ca2 +, Ba2 +) on adsorption of model proteins (lysozyme and bovine serum albumin). The localizations of both proteins and their conformational modifications in the interlayer space of the natural clay mineral were studied by X-ray diffraction, transmission electronic microscopy and fluorescence experiments. Based on Langmuir model isotherms, a strong influence of interlayer cation on maximum adsorbed amount and adsorption equilibrium constant has been observed. However the usual kosmotrope/chaotrope classification cannot be used to describe protein adsorption.
Fichier principal
Vignette du fichier
Manuscript_Lepoitevin-corrected.pdf (397.74 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

insu-01017005 , version 1 (01-07-2014)

Identifiers

Cite

Mathilde Lepoitevin, Maguy Jaber, Régis Guégan, Jean-Marc Janot, Philippe Dejardin, et al.. BSA and lysozyme adsorption on homoionic montmorillonite: Influence of the interlayer cation. Applied Clay Science, 2014, 95, pp.396-402. ⟨10.1016/j.clay.2014.05.003⟩. ⟨insu-01017005⟩
412 View
918 Download

Altmetric

Share

Gmail Facebook X LinkedIn More