Phenotypic and genotypic within-host diversity of Pseudomonas aeruginosa urinary isolates - INSU - Institut national des sciences de l'Univers Access content directly
Journal Articles Scientific Reports Year : 2022

Phenotypic and genotypic within-host diversity of Pseudomonas aeruginosa urinary isolates

Abstract

This study aimed to assess phenotypic and molecular inter-patient and within-host diversity of Pseudomonas aeruginosa isolates responsible for urinary tract infection (UTI) or asymptomatic bacteriuria (AB). Clinical data of 120 consecutive P. aeruginosa UTI (n = 40) and AB (n = 80) were prospectively analyzed. Up to five P. aeruginosa isolates per sample were collected. Antimicrobial susceptibility testing (AST) was determined for all isolates (n = 591); a subset of 358 was characterized by multilocus sequence typing. 444 isolates (75%) were non-multidrug resistant (MDR), 113 (19%) were MDR, and 34 (6%) were extensively drug resistant. A genetically highly diverse population was observed (64 sequence types [STs]), without strict correlation between genotypes and clinical settings. 35 patients (28%; 12 UTIs and 23 ABs) presented distinct antimicrobial resistance (AMR) profiles within a given urine sample, significantly associated with previous carbapenem and fluroquinolones exposure; five of them also exhibited polyclonal UTI or AB (with isolates belonging to two STs). P. aeruginosa urinary isolates of these 120 patients were highly diverse, in terms of AMR as well as genetic background. Both within-host AMR and molecular diversity can complicate AST, treatment and control of P. aeruginosa UTI.
Fichier principal
Vignette du fichier
s41598-022-09234-5.pdf (1.73 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

insu-03665281 , version 1 (11-05-2022)

Licence

Attribution

Identifiers

Cite

Agnès Cottalorda, Sandrine Dahyot, Anaïs Soares, Kevin Alexandre, Isabelle Zorgniotti, et al.. Phenotypic and genotypic within-host diversity of Pseudomonas aeruginosa urinary isolates. Scientific Reports, 2022, 12, ⟨10.1038/s41598-022-09234-5⟩. ⟨insu-03665281⟩
27 View
45 Download

Altmetric

Share

Gmail Facebook X LinkedIn More