The restrictions imposed on human activities during the COVID-19 lockdown (LD) period provided a new scenario to identify potential sources of atmospheric pollution. There are several studies that deal with changes in air quality around the world during LD, but very few on atmospheric NH3. Therefore, we examine the changes in global NH3 during LD (April–May 2020) in comparison to the pre-lockdown (PreLD, April–May 2017–2019) and post-lockdown (PostLD, April–May 2021) periods, and assess the factors responsible for these changes. We observe an increase in NH3 during LD across the latitudes, with very high values in the western Europe, Eastern China (EC), Indian subcontinent and the eastern United States of America (USA). However, a decline in NH3 is observed in some regions of South America (SA) and North America (NA). Similar changes in NH3 during LD is also observed in smaller spatial scales, as found in 3000 cities across the globe. The reduction of sulphur dioxide (SO2), nitrogen dioxide (NO2), nitric acid (HNO3), humidity and cloud cover as a result of restrictions on human activities, particularly in the western Europe and the USA, may have impeded the conversion of NH3 to particulates, which led to a higher NH3 there. Nevertheless, agricultural activities and livestock are the most prominent sources of atmospheric NH3, which were not under restriction during LD, show enhancement in terms of the use of nitrogen fertilizer, crop production and area harvested, which also cause a rise in NH3 in these regions. Therefore, it is evident that the changes in meteorology and atmospheric composition, together with increased agricultural activities, led to the global increase in atmospheric NH3 during LD. That is, most pollutants show a decline during LD, but NH3 exhibits a rise due to its sources such as agricultural activities. Henceforth, environmental regulations, policies and advanced technologies are required in the agricultural sector, such as fertilizer deep placement using urea briquettes and restricted application of nitrogen fertilizers, to curb NH3 emissions.