Fluctuations of the Charquini glaciers (Cordillera Real, Bolivia) have been reconstructed for the Little Ice Age (LIA) from a set of 10 moraines extending below the present glacier termini. A lichenometric method using the Rhizocarpon geographicum was used to date the moraines and reconstruct the main glacier fluctuations over the period. The maximum glacier extent occurred in the second half of the 17th century, followed by nearly continuous retreat with three interruptions during the 18th and the 19th centuries, marked by stabilisation or minor advances. Results obtained in the Charquini area are first compared with other dating performed in the Peruvian Cordillera Blanca and then with the fluctuations of documented glaciers in the Northern Hemisphere. Glacier fluctuations along the tropical Andes (Bolivia and Peru) were in phase during the LIA and the solar forcing appears to be important during the period of glacier advance. Compared with the Northern Hemisphere mid-latitudes, the major advance observed on these glaciers during the first half of the 19th century is not present in the tropical Andes. This discrepancy may be due to regional scale climate variations.