The Albian organic-rich successions of the lower part of the Fahdene Formation (Albian to Cenomanian, Tunisia) were studied using sedimentology (analysis of carbonate contents and observation of thin sections), bulk organic geochemistry (Rock-Eval pyrolysis), and molecular biomarker distributions. The selected outcrops cover different structural domains from western central Tunisia (Jebel Hamra) to the Diapir zone or the Tunisian Trough (Koudiat Berkouchia, Jebel Ghazouan and Ain Asker from the southwest to the northeast, respectively). Total Organic Carbon values between 0.17 and 3.43% evidenced the record of the Albian oceanic anoxic events OAE1b and OAE1c in this southern Tethys margin. Rock-Eval data and molecular biomarkers (n-alkanes, steranes and terpanes) revealed thermally mature organic matter (OM) consisting of a mixed Type II/III OM (marine planktonic and continental origins). The abundant tricyclic terpanes, C30 17α(H) Diahopanes, and the regular decrease of homohopanes (C31 to C35) in the terpane series, were also indicative of a high contribution of bacterial OM that had been developed under stagnant dysoxic/anoxic environments.